TAE - A Classic Electrophoresis Buffer

TAE is available  commercially but can readily  be made in the Biotechnology  lab. Here is the recipe for a 50X stock.

To make 1 litre :
 Add  Tris base (242g) to  57 mL of Acetic Acid  and 100ml of 0.5M EDTA.  Q to 1 litre with dH2O .