Capital Loading Dye - Native Gel Electrophoresis

Sustainability is key to the running of Biotechnology courses. Paradoxically,  it's the small items that consume the most. One staple in the lab, is Native Gel loading dye which is used in  native gel electrophoresis  of DNA, ssRNA and Proteins.  Here is a reliable recipe  to make enough gel loading dye for a semester .

Fill up a  15ml  tube with  glycerol to  5ml , add  BPB (2ml of 1% ) , XC (2ml of 1% ) and EDTA ( 20uL  of 500mM) . Q with dH20  to 10 ml and aliquot  ( 100ul ). This provides a 10X loading dye.

The attached picture shows  plasmid DNA and ladder markers run  on a  1% agarose gel electrophoresis  in 1XTAE buffer using Capital Loading Dye. Image captured with iPhone .