The Bradford Reagent for Protein Determination

The Bradford Reagent  is the current  standard for protein determination, but it is quite expensive .   Most educational  exercises still use  the Biuret method since it is much more economical. However, the Bradford method is the one that  most  Biotechnology graduates  will  use in the workplace and so  it is important  to use this reagent in Biotechnology technique courses.

 Here, we describe  how to make  Bradford reagent  in economical fashion  from  readily available laboratory reagents.

Add Commassie brilliant blue ( 50mg) to  Methanol (50ml) , then  slowly add  100ml of phosporic acid , Q to 1 litre with dH20 .